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  • BSI
    BS EN 12823-1:2014 Foodstuffs. Determination of vitamin A by high performance liquid chromatography - Measurement of all-E-retinol and 13-Z-retinol
    Edition: 2014
    $313.97
    / user per year

Description of BS EN 12823-1:2014 2014

This European Standard specifies a method for the determination of vitamin A in foodstuffs by high performance liquid chromatography (HPLC). This method has been validated in an interlaboratory study with samples of margarine and milk powder with all-E-retinol levels ranging from 653 µg/100 g to 729 µg/100 g and with 13-Z-retinol levels ranging from 30 µg/100 g to 39 µg/100 g. The determination of vitamin A content is carried out by the measurement of all-E-retinol, 13-Z-retinol and β-carotene. This part covers the measurement of all-E-retinol and 13-Z-retinol.

The extract obtained after saponification in this method can be used for the determination of β-carotene, as described in EN 12823-2:2000, Foodstuffs - Determination of vitamin A by high performance liquid chromatography - Part 2: Measurements of β-carotene. In this case, the saponification temperature should preferably not exceed 80 °C in order to prevent isomerisation and oxidation of β-carotene.



Standard NumberBS EN 12823-1:2014
TitleFoodstuffs. Determination of vitamin A by high performance liquid chromatography - Measurement of all-E-retinol and 13-Z-retinol
StatusConfirmed
Publication Date2014-05-31
ReplacesBS EN 12823-1:2000
Identical National Standard OfEN 12823-1:2014
DescriptorsVitamins, Testing conditions, Determination of content, Chemical analysis and testing, Vitamin A, Food testing, Chromatography, Food products, Liquid chromatography, Precision
ICS67.050 General methods of tests and analysis for food products
CommitteeAW/275
ISBN978 0 580 77938 1
PublisherBSI
Pages20

About BSI

BSI Group, also known as the British Standards Institution is the national standards body of the United Kingdom. BSI produces technical standards on a wide range of products and services and also supplies certification and standards-related services to businesses.
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