1. Scope

1.1 This test guide is designed to give general guidance for assessing the potential phytotoxicity of water soluble test material to freshwater emergent macrophytes.

1.2 This renewal test continuously exposes selected plant species, growing in sediment, to various concentrations of test material, dissolved in a nutrient solution.

1.3 This test guide is based on the Toxic Substances Control Act (TSCA) guidelines for conducting toxicity tests with terrestrial plants and is applicable to most water soluble chemicals , either individually or in formulations, commercial products, or known mixtures (see Guides E 1193 and E 1598). With slight modifications the procedure also might be used for effluents (see Guide E 1192).

1.4 Results from this toxicity test can be used to report an EC50 or NOEC (see Section 3) based on the concentration of chlorophyll extracted from the plants (see Guides D 3731 and E 1218). In some situations, it might be necessary to only test at one concentration to determine whether or not that specific concentration is toxic to the plants.

2. Referenced Documents (purchase separately) The documents listed below are referenced within the subject standard but are not provided as part of the standard.

ASTM Standards

D3731 Practices for Measurement of Chlorophyll Content of Algae in Surface Waters

E729 Guide for Conducting Acute Toxicity Tests on Test Materials with Fishes, Macroinvertebrates, and Amphibians

E943 Terminology Relating to Biological Effects and Environmental Fate

E1023 Guide for Assessing the Hazard of a Material to Aquatic Organisms and Their Uses

E1192 Guide for Conducting Acute Toxicity Tests on Aqueous Ambient Samples and Effluents with Fishes, Macroinvertebrates, and Amphibians

E1193 Guide for Conducting Daphnia magna Life-Cycle Toxicity Tests

E1218 Guide for Conducting Static Toxicity Tests with Microalgae

E1391 Guide for Collection, Storage, Characterization, and Manipulation of Sediments for Toxicological Testing and for Selection of Samplers Used to Collect Benthic Invertebrates

E1598 Practice for Conducting Early Seedling Growth Tests

E1706 Test Method for Measuring the Toxicity of Sediment-Associated Contaminants with Freshwater Invertebrates

E1733 Guide for Use of Lighting in Laboratory Testing

Keywords

chlorophyll; emergent macrophyte; phytotoxicity test; surrogate species ;

ICS Code

ICS Number Code 07.100.20 (Microbiology of water); 13.060.70 (Examination of water for biological properties)

DOI: 10.1520/E1841-96

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