Cart (0)
  • No items in cart.
Total
$0
There is a technical issue about last added item. You can click "Report to us" button to let us know and we resolve the issue and return back to you or you can continue without last item via click to continue button.
Filters:
FORMAT
EDITION
to
PUBLISHER
(1)
(337)
(585)
(54)
(234)
(996)
(657)
(2154)
(117)
(94142)
(54)
(560)
(124)
(33)
(21)
(20)
(94146)
(3)
(17)
(1)
(374)
(309)
(6631)
(241)
(16)
(6)
(1646)
(17)
(19)
(28)
(4)
 
(6)
(7)
(115)
(3)
(57)
(5)
(5)
(1)
(1)
(2)
(25)
(27)
(27)
(13)
(61)
(24)
(22)
(7)
(8)
(20)
(1)
(3)
(50)
(6)
(31)
CONTENT TYPE
 Act
 Admin Code
 Announcements
 Bill
 Book
 CADD File
 CAN
 CEU
 Charter
 Checklist
 City Code
 Code
 Commentary
 Comprehensive Plan
 Conference Paper
 County Code
 Course
 DHS Documents
 Document
 Errata
 Executive Regulation
 Federal Guideline
 Firm Content
 Guideline
 Handbook
 Interpretation
 Journal
 Land Use and Development
 Law
 Legislative Rule
 Local Amendment
 Local Code
 Local Document
 Local Regulation
 Local Standards
 Manual
 Model Code
 Model Standard
 Notice
 Ordinance
 Other
 Paperback
 PASS
 Periodicals
 PIN
 Plan
 Policy
 Product
 Product - Data Sheet
 Program
 Provisions
 Requirements
 Revisions
 Rules & Regulations
 Standards
 State Amendment
 State Code
 State Manual
 State Plan
 State Standards
 Statute
 Study Guide
 Supplement
 Sustainability
 Technical Bulletin
 All
  • ASTM
    D6499-12 Standard Test Method for The Immunological Measurement of Antigenic Protein in Natural Rubber and its Products
    Edition: 2012
    $93.60
    Unlimited Users per year

Description of ASTM-D6499 2012

ASTM D6499 - 12

Standard Test Method for The Immunological Measurement of Antigenic Protein in Natural Rubber and its Products

Active Standard ASTM D6499 | Developed by Subcommittee: D11.40

Book of Standards Volume: 09.02




ASTM D6499

Significance and Use

Type 1 latex allergy most commonly manifests as localized urticaria after contact of skin with natural rubber but can also include symptoms of allergic rhinoconjunctivitis, asthma and rarely anaphylaxis. This immediate (Type I) allergy is caused by natural proteins inherent to the rubber tree, which remain on the finished natural rubber products. The quantification of protein levels in NRL products using the standard colorimetric protein assays may give spurious results due to chemical additives in the latex formulations that interfere with the assay (2,3) . Furthermore, the amount of protein found in NRL products are often below the detection limits of the standard colorimetric protein assay (4,5) .

This test method describes an immunological method for quantitation of natural rubber latex proteins using rabbit anti-NRL serum. Rabbits immunized with NRL proteins react to the majority of the proteins present, and their sera have the capability to detect most if not all of the proteins in NRL. Therefore, although rabbit antibody reacts with antigenic material, this should not be considered as quantitative measure of total protein levels.

1. Scope

1.1 This test method covers an immunological method to determine the amount of antigenic protein in natural rubber and its products using rabbit antisera specific for natural rubber latex (NRL) proteins. This immunoassay procedure quantitatively measures the level of antigenic latex proteins in solution using an inhibition format. The samples may include glove or other rubber product extracts which have been collected in order to measure the latex protein levels. Although this method detects antigenic proteins, it should not be considered as a measure of allergenic proteins. Correlation of protein/antigen levels with the level of allergenic proteins has not been fully established.

1.2 For the purpose of this test method, the range of protein will be measured in terms of microgram to milligram quantities.

1.3 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.


2. Referenced Documents (purchase separately) The documents listed below are referenced within the subject standard but are not provided as part of the standard.

ASTM Standards

D4483 Practice for Evaluating Precision for Test Method Standards in the Rubber and Carbon Black Manufacturing Industries

D5712 Test Method for Analysis of Aqueous Extractable Protein in Natural Rubber and Its Products Using the Modified Lowry Method

E691 Practice for Conducting an Interlaboratory Study to Determine the Precision of a Test Method


Keywords

ELISA; natural rubber laytex; proteins;


ICS Code

ICS Number Code 11.100 (Laboratory medicine)


DOI: 10.1520/D6499-12

ASTM International is a member of CrossRef.

ASTM D6499

The following editions for this book are also available...

This book also exists in the following packages...

Year Publisher Title Annual Price
VAR
ASTM
[+] $727.42 Buy
VAR
ASTM
[+] $1,434.68 Buy

Subscription Information

MADCAD.com ASTM Standards subscriptions are annual and access is unlimited concurrency based (number of people that can access the subscription at any given time) from single office location. For pricing on multiple office location ASTM Standards Subscriptions, please contact us at info@madcad.com or +1 800.798.9296.

 

Some features of MADCAD.com ASTM Standards Subscriptions are:

- Online access: With MADCAD.com’ s web based subscription service no downloads or installations are required. Access ASTM Standards from any browser on your computer, tablet or smart phone.

- Immediate Access: As soon as the transaction is completed, your ASTM Standards Subscription will be ready for access.

 

For any further information on MADCAD.com ASTM Standards Subscriptions, please contact us at info@madcad.com or +1 800.798.9296.

 

About ASTM

ASTM International, formerly known as the American Society for Testing and Materials (ASTM), is a globally recognized leader in the development and delivery of international voluntary consensus standards. Today, some 12,000 ASTM standards are used around the world to improve product quality, enhance safety, facilitate market access and trade, and build consumer confidence. ASTM’s leadership in international standards development is driven by the contributions of its members: more than 30,000 of the world’s top technical experts and business professionals representing 150 countries. Working in an open and transparent process and using ASTM’s advanced electronic infrastructure, ASTM members deliver the test methods, specifications, guides, and practices that support industries and governments worldwide.

GROUPS