ASTM D6499-00
Historical Standard: ASTM D6499-00 Standard Test Method for the Immunological Measurement of Antigenic Protein in Natural Rubber and its Products
SUPERSEDED (see Active link, below)
ASTM D6499
1. Scope
1.1 This test method covers an immunological method to determine the amount of antigenic protein in natural rubber and its products using rabbit antisera specific for natural rubber latex (NRL) proteins. This immunoassay procedure quantitatively measures the level of antigenic latex proteins in solution using an inhibition format. The samples may include glove or other rubber product extracts which have been collected in order to measure the latex protein levels. Although this method detects antigenic proteins, it should not be considered as a measure of allergenic proteins. Correlation of protein/antigen levels with the level of allergenic proteins has not been fully established.
1.2 For the purpose of this test method, the range of protein will be measured in terms of microgram to milligram quantities.
This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.
2. Referenced Documents (purchase separately) The documents listed below are referenced within the subject standard but are not provided as part of the standard.
ASTM Standards
D4483 Practice for Evaluating Precision for Test Method Standards in the Rubber and Carbon Black Manufacturing Industries
D5712 Test Method for Analysis of Aqueous Extractable Protein in Natural Rubber and Its Products Using the Modified Lowry Method
E691 Practice for Conducting an Interlaboratory Study to Determine the Precision of a Test Method
Keywords
ELISA; natural rubber laytex; proteins;
ICS Code
ICS Number Code 11.100 (Laboratory medicine)
DOI: 10.1520/D6499-00
ASTM International is a member of CrossRef.
