ASTM D5712-99e1
Historical Standard: ASTM D5712-99e1 Standard Test Method for Analysis of Aqueous Extractable Protein in Natural Rubber and Its Products Using the Modified Lowry Method
SUPERSEDED (see Active link, below)
ASTM D5712
1. Scope
1.1 This test method covers an analytical test for determining the amount of total aqueous extractable protein associated with natural rubber (NR) and its products. Water soluble proteins are extracted in a buffer solution and then precipitated to concentrate them and also to separate them from water soluble substances which may interfere with the determination. The extracted protein is redissolved and quantified colorimetrically by the modified Lowry method using a protein standard.
1.2 For the purpose of this test method, the range of protein measurement will be based on the limit of detection and quantitation and recorded in micrograms per dm 2 NR specimen.
1.3 The test method is designed to be accurate and compatible with the industrial environment.
1.4 Steps are included in this test method to minimize the effects of interfering substances.
1.5 It is recognized that other methods for the analysis of leachable proteins exist and these may be used for routine quality control purposes provided they have been validated and a correlation established against the reference method specified by this Standard.
1.6 The values stated in SI units are to be regarded as the standard.
1.7 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.
2. Referenced Documents (purchase separately) The documents listed below are referenced within the subject standard but are not provided as part of the standard.
ASTM Standards
D3577 Specification for Rubber Surgical Gloves
D3578 Specification for Rubber Examination Gloves
D4483 Practice for Evaluating Precision for Test Method Standards in the Rubber and Carbon Black Manufacturing Industries
Keywords
amino acid analysis; Lowry; natural rubber latex; protein; Colorimetric analysis; Natural rubber (NR); Protein; Spectrophotometry; Total water extractable protein ;
ICS Code
ICS Number Code 83.040.10 (Latex and raw rubber)
DOI: 10.1520/D5712-99E01
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