ASTM D5712-99
Historical Standard: ASTM D5712-99 Standard Test Method for the Analysis of Aqueous Extractable Protein in Natural Rubber and Its Products Using the Modified Lowry Method
SUPERSEDED (see Active link, below)
ASTM D5712
1. Scope
1.1 This test method provides an analytical test for determining the amount of total water extractable protein associated with natural rubber (NR) and its products. The test method involves an extraction and precipitation procedure followed by an assay of protein content.
1.2 For the purpose of this test method, the range of protein will be measured in terms of microgram to milligram quantities.
1.3 The test method is designed to be accurate and compatible with the industrial environment.
1.4 Steps are included in this test method to minimize the effects of interfering substances.
1.5 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use. For specific hazard statements, see Section 9.
2. Referenced Documents (purchase separately) The documents listed below are referenced within the subject standard but are not provided as part of the standard.
ASTM Standards
D3577 Specification for Rubber Surgical Gloves
D3578 Specification for Rubber Examination Gloves
D4483 Practice for Evaluating Precision for Test Method Standards in the Rubber and Carbon Black Manufacturing Industries
Keywords
amino acid analysis; Lowry; natural rubber latex; protein; Colorimetric analysis; Natural rubber (NR); Protein; Spectrophotometry; Total water extractable protein ;
ICS Code
ICS Number Code 83.040.10 (Latex and raw rubber)
DOI: 10.1520/D5712-99
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