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BS EN ISO 17601:2018 Soil quality. Estimation of abundance of selected microbial gene sequences by quantitative PCR from DNA directly extracted from soil >
BS EN ISO 17601:2018 Soil quality. Estimation of abundance of selected microbial gene sequences by quantitative PCR from DNA directly extracted from soil, 2018
- undefined
- European foreword
- Endorsement notice
- Foreword
- Introduction
- 1 Scope
- 2 Normative references
- 3 Terms and definitions
- 4 Principle
- 5 Test materials
- 5.1 DNA
- 5.2 Bacteria
- 5.3 Plasmid
- 5.4 Enzyme
- 5.5 Chemicals
- 5.6 Product for bacterial culture medium
- 5.7 Buffer and reagents
- 6 Apparatus
- 7 Procedure
- 7.1 qPCR standard preparation and calibration of qPCR assay (task 1)
- 7.1.1 General
- 7.1.2 Amplicon design (task 1, step 1)
- 7.1.3 qPCR standard preparation (task 1, step 2)
- 7.1.4 Isolate DNA, environmental DNA, artificial DNA
- 7.1.5 Calibration of the qPCR (task 1, step 3)
- 7.2 Preparation of soil DNA template and inhibition test (task 2)
- 7.2.1 General
- 7.2.2 Soil DNA preparation (task 2, step 4)
- 7.2.3 Inhibition test (task 2, step 5)
- 7.2.4 Dilution of DNA template
- 7.3 qPCR assay (task 3)
- 7.3.1 General
- 7.3.2 qPCR
- 7.4 Validation and analysis of qPCR assay (task 4)
- 7.4.1 General
- 7.4.2 Validation of the qPCR assay
- 7.4.3 Calculation of the copy number of the gene of interest in the soil DNA extract
- 8 Examination of the critical steps of the qPCR assay
- 9 Expression of the results of the qPCR assay
- 10 International ring test
- 11 Test report
- Annex A (informative) Description of principal steps of TaqMan® qPCR assay
- Annex B (informative) International ring-test for evaluating qPCR to quantify the abundance of selected microbial gene sequences from DNA directly extracted from soil
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