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Description of ASTM-E2146 2001ASTM E2146-01Withdrawn Standard: Standard Guide for Detection of Nucleic Acid Sequences of the Human Immunodeficiency Virus HIV-1 by the Polymerase Chain Reaction Technique (Withdrawn 2010)ASTM E2146Scope 1.1 This guide covers considerations, criteria, principles and recommendations that should be helpful when developing, utilizing, or assessing PCR protocols to amplify and detect DNA (by PCR) or RNA [by reverse transcriptase PCR (RT-PCR)] from HIV. This guide is not a specific protocol for the detection of HIV. It is intended to provide information that will assist the user in obtaining quality and reliable data. The guide is closely related to and should be used concurrently with the general PCR guideline E 1873. 1.2 This guide has been developed for use in any molecular biology or biotechnology laboratory. This includes but is not limited to clinical and diagnostic laboratories involved with HIV detection. 1.3 This guide does not cover details of the various methods such as gel electrophoresis, that can be utilized to identify PCR-amplified HIV nucleic acid sequences, nor does it cover details of instrument (thermocycler) calibration. 1.4 This guide does not cover specific variations of the basic PCR or RT-PCR technology (e.g., quantitative PCR, multiplex PCR and in situ PCR). 1.5 This guide does not address the additional considerations necessary for the performance and validation of a quantitative PCR test for HIV. Note 1—Warning: Laboratory work involving certain clinical specimens and microorganisms can be hazardous to personnel. Note 2—Precaution: Biosafety Level 2 facilities are recommended for potentially hazardous work (2), and many laboratories use Biosafety Level 3 facilities when working with HIV. Safety guidelines should be adhered to according to NCCLS M29-A and other recommendations (2). Keywords AIDS; amplification; DNA primer; DNA probe; HIV; human immunodeficiency virus; PCR; polymerase chain reaction ICS Code ICS Number Code 11.100 (Laboratory medicine) DOI: 10.1520/E2146-01 This book also exists in the following packages...Subscription InformationMADCAD.com ASTM Standards subscriptions are annual and access is unlimited concurrency based (number of people that can access the subscription at any given time) from single office location. For pricing on multiple office location ASTM Standards Subscriptions, please contact us at info@madcad.com or +1 800.798.9296.
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About ASTMASTM International, formerly known as the American Society for Testing and Materials (ASTM), is a globally recognized leader in the development and delivery of international voluntary consensus standards. Today, some 12,000 ASTM standards are used around the world to improve product quality, enhance safety, facilitate market access and trade, and build consumer confidence. ASTM’s leadership in international standards development is driven by the contributions of its members: more than 30,000 of the world’s top technical experts and business professionals representing 150 countries. Working in an open and transparent process and using ASTM’s advanced electronic infrastructure, ASTM members deliver the test methods, specifications, guides, and practices that support industries and governments worldwide. |
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