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BS EN ISO 6785:2007 Milk and milk products. Detection of Salmonella spp., 2008
- Milk and milk products�— Detection of
- Contents Page
- Foreword
- Milk and milk products�— Detection of [Go to Page]
- 1��� Scope
- 2��� Normative reference
- 3��� Terms and definitions
- 4��� Principle [Go to Page]
- 4.1��� General
- 4.2��� Pre-enrichment in non-selective liquid medium
- 4.3��� Enrichment in selective liquid media
- 4.4��� Streaking out and recognition
- 4.5��� Confirmation
- 5��� Culture media, reagents and sera [Go to Page]
- 5.1��� Water
- 5.2��� Culture media [Go to Page]
- 5.2.1��� Pre-enrichment medium: Buffered peptone water [Go to Page]
- 5.2.1.1��� Composition
- 5.2.1.2��� Preparation
- 5.2.2��� First selective enrichment medium: Rappaport-Vassiliadis modified, magnesium chloride/ma... [Go to Page]
- 5.2.2.1��� Solution�A [Go to Page]
- 5.2.2.1.1��� Composition
- 5.2.2.1.2��� Preparation
- 5.2.2.2��� Solution�B [Go to Page]
- 5.2.2.2.1��� Composition
- 5.2.2.2.2��� Preparation
- 5.2.2.3��� Solution�C [Go to Page]
- 5.2.2.3.1��� Composition
- 5.2.2.3.2��� Preparation
- 5.2.2.4��� Complete medium [Go to Page]
- 5.2.2.4.1��� Composition
- 5.2.2.4.2��� Preparation
- 5.2.3��� Second selective enrichment medium: Selenite/cystine medium [Go to Page]
- 5.2.3.1��� Base [Go to Page]
- 5.2.3.1.1��� Composition
- 5.2.3.1.2��� Preparation
- 5.2.3.2��� l [Go to Page]
- 5.2.3.2.1��� Composition
- 5.2.3.2.2��� Preparation
- 5.2.3.3��� Complete medium [Go to Page]
- 5.2.3.3.1��� Composition
- 5.2.3.3.2��� Preparation
- 5.2.4��� First selective solid medium: Brilliant green/phenol red agar (Edel and Kampelmacher) [Go to Page]
- 5.2.4.1��� Base [Go to Page]
- 5.2.4.1.1��� Composition
- 5.2.4.1.2��� Preparation
- 5.2.4.2��� Sugar/phenol red solution [Go to Page]
- 5.2.4.2.1��� Composition
- 5.2.4.2.2��� Preparation
- 5.2.4.3��� Brilliant green solution [Go to Page]
- 5.2.4.3.1��� Composition
- 5.2.4.3.2��� Preparation
- 5.2.4.4��� Complete medium [Go to Page]
- 5.2.4.4.1��� Composition
- 5.2.4.4.2��� Preparation
- 5.2.4.4.3��� Preparation of the agar plates
- 5.2.5��� Second selective solid medium
- 5.2.6��� Nutrient agar [Go to Page]
- 5.2.6.1��� Composition
- 5.2.6.2��� Preparation
- 5.2.6.3��� Preparation of agar plates
- 5.2.7��� Triple sugar/iron agar (TSI agar) [Go to Page]
- 5.2.7.1��� Composition
- 5.2.7.2��� Preparation
- 5.2.8��� Urea agar (Christensen) [Go to Page]
- 5.2.8.1��� Base [Go to Page]
- 5.2.8.1.1��� Composition
- 5.2.8.1.2��� Preparation
- 5.2.8.2��� Urea solution [Go to Page]
- 5.2.8.2.1��� Composition
- 5.2.8.2.2��� Preparation
- 5.2.8.3��� Complete medium [Go to Page]
- 5.2.8.3.1��� Composition
- 5.2.8.3.2��� Preparation
- 5.2.9��� l [Go to Page]
- 5.2.9.1��� Composition
- 5.2.9.2��� Preparation
- 5.3��� Reagents [Go to Page]
- 5.3.1��� Saline solution [Go to Page]
- 5.3.1.1��� Composition
- 5.3.1.2��� Preparation
- 5.3.2��� Reagents for [Go to Page]
- 5.3.2.1��� Toluene
- 5.3.2.2��� Buffer solution [Go to Page]
- 5.3.2.2.1��� Composition
- 5.3.2.2.2��� Preparation
- 5.3.2.3��� ONPG solution [Go to Page]
- 5.3.2.3.1��� Composition
- 5.3.2.3.2��� Preparation
- 5.3.2.4��� Complete reagent [Go to Page]
- 5.3.2.4.1��� Composition
- 5.3.2.4.2��� Preparation
- 5.3.3��� Reagents for Voges-Proskauer (VP) reaction [Go to Page]
- 5.3.3.1��� VP medium [Go to Page]
- 5.3.3.1.1��� Composition
- 5.3.3.1.2��� Preparation
- 5.3.3.2��� Creatine solution ( [Go to Page]
- 5.3.3.2.1��� Composition
- 5.3.3.2.2��� Preparation
- 5.3.3.3��� 1-Naphthol, ethanolic solution [Go to Page]
- 5.3.3.3.1��� Composition
- 5.3.3.3.2��� Preparation
- 5.3.3.4��� Potassium hydroxide solution [Go to Page]
- 5.3.3.4.1��� Composition
- 5.3.3.4.2��� Preparation
- 5.3.4��� Reagents for indole reaction [Go to Page]
- 5.3.4.1��� Tryptone/tryptophan medium [Go to Page]
- 5.3.4.1.1��� Composition
- 5.3.4.1.2��� Preparation
- 5.3.4.2��� Kovac’s reagent [Go to Page]
- 5.3.4.2.1��� Composition
- 5.3.4.2.2��� Preparation
- 5.3.5��� Semi-solid nutrient agar [Go to Page]
- 5.3.5.1��� Composition
- 5.3.5.2��� Preparation
- 5.3.5.3��� Preparation of agar plates
- 5.4��� Sera
- 6��� Apparatus and glassware [Go to Page]
- 6.1��� Apparatus for wet sterilization (autoclave)
- 6.2��� Oven
- 6.3��� Incubator
- 6.4��� Water bath
- 6.5��� Water baths
- 6.6��� Loops
- 6.7��� pH-meter
- 6.8��� Culture bottles
- 6.9��� Culture tubes
- 6.10��� Measuring cylinders
- 6.11��� Graduated pipettes
- 6.12��� Petri dishes
- 7��� Sampling
- 8��� Preparation of test sample
- 9��� Procedure [Go to Page]
- 9.1��� Safety precautions
- 9.2��� Test portion and pre-enrichment [Go to Page]
- 9.2.1��� General
- 9.2.2��� Raw milk, heat-treated milk and liquid milk products
- 9.2.3��� Dried milk products
- 9.2.4��� Lactose
- 9.2.5��� Casein, caseinates, cheese
- 9.2.6��� Butter
- 9.2.7��� Frozen milk products (including edible ices)
- 9.2.8��� Fermented milks, yoghurt, custards, desserts
- 9.3��� Enrichment [Go to Page]
- 9.3.1��� Non-selective pre-enrichment
- 9.3.2��� Selective enrichment [Go to Page]
- 9.3.2.1��� Transfer
- 9.3.2.2��� Incubate the inoculated RVS medium (
- 9.4��� Streaking out and recognition [Go to Page]
- 9.4.1��� Using the culture obtained in the RVS medium (
- 9.4.2��� Using the culture obtained in the selenite/cystine medium (
- 9.4.3��� Incubate the plates (bottom uppermost) in the incubator (
- 9.4.4��� After incubating the RVS medium and the selenite/cystine medium for a further
- 9.4.5��� After each incubation (
- 9.4.6��� On brilliant green/phenol red agar (
- 9.5��� Confirmation [Go to Page]
- 9.5.1��� Selection of colonies for confirmation
- 9.5.2��� Incubation
- 9.5.3��� Biochemical confirmation [Go to Page]
- 9.5.3.1��� General
- 9.5.3.2��� Triple sugar/iron agar (
- 9.5.3.3��� Urea agar (
- 9.5.3.4��� l
- 9.5.3.6��� Voges-Proskauer reaction (
- 9.5.3.7��� Indole reaction (
- 9.5.3.8��� Interpretation of the biochemical tests
- 9.5.4��� Commercially diagnostic systems
- 9.5.5��� Serological confirmation [Go to Page]
- 9.5.5.1��� General
- 9.5.5.2��� Elimination of auto-agglutinable strains
- 9.5.5.3��� Examination for O-antigens
- 9.5.5.4��� Examination for Vi-antigens
- 9.5.5.5��� Examination for H-antigens
- 9.5.5.6��� Interpretation of serological reactions
- 9.5.6��� Interpretation of biochemical and serological reactions
- 9.5.7��� Definitive confirmation
- 10��� Control cultures
- 11��� Expression of results
- 12��� Safety precautions [Go to Page]
- 12.1��� The procedure specified in this International Standard shall only be carried out in labor...
- 12.2��� These procedures shall not be performed in quality control laboratories, or in food manuf...
- 12.3��� Full bacteriological precautions shall be taken at all times whilst carrying out the proc...
- 12.4��� Special care should be taken with the laboratory use of selenite solutions because of the...
- 13��� Test report
- Diagram of procedure
- Specification for brilliant green [Go to Page]
- B.1��� Bacteriological performance
- B.2��� Test method [Go to Page]
- B.2.1��� Medium
- B.2.2��� Procedure
- Standard method for streaking agar plates
- Bibliography [Go to Page]