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BS EN 16187:2015 Foodstuffs. Determination of fumonisin B1 and fumonisin B2 in processed maize containing foods for infants and young children. HPLC method with immunoaffinity column cleanup and fluorescence detection after pre-column derivatisation >
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BS EN 16187:2015 Foodstuffs. Determination of fumonisin B1 and fumonisin B2 in processed maize containing foods for infants and young children. HPLC method with immunoaffinity column cleanup and fluorescence detection after pre-column derivatisation, 2015
- Foreword
- 1 Scope
- 2 Normative references
- 3 Principle
- 4 Reagents
- Table 1 — Preparation of mixed FBs calibration solutions for HPLC
- 5 Apparatus
- Table 2 — Gradient conditions
- 6 Procedure [Go to Page]
- 7 Calculation
- 8 Precision [Go to Page]
- 9 Test report
- Annex A (informative) Typical chromatograms
- Figure A.1 — Typical chromatogram of baby food (pre-cooked flours of diastased maize and tapioca) spiked with 250,0 µg/kg fumonisin B1 (FB1) and 62,5 µg/kg fumonisin B2 (FB2)
- Figure A.2 — Typical chromatogram of baby food (biscuits containing wheat, barley, rye, rice and maize) naturally contaminated with 164,8 µg/kg FB1 and 35,6 µg/kg FB2
- Annex B (informative) Precision data
- Table B.1 — Precision and recovery data for fumonisins B1+B2 in baby food
- Table B.2 — Precision and recovery data for fumonisin B1 in baby food
- Table B.3 — Precision and recovery data for fumonisin B2 in baby food
- Annex C (informative) Comparison between the method in this document and EN 14352:2004 and EN 13585:2001 on fumonisins in maize
- Table C.1 — Comparison between the method in this document and EN 14352:2004 and EN 13585:2001
- Bibliography [Go to Page]